Composite

Part:BBa_K3040007:Design

Designed by: TSAI, CHIA-TSEN   Group: iGEM19_NTHU_Taiwan   (2019-10-18)


placUV5-R0010-TesA_pFadBA- RBS -mRFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1106
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1107
    Illegal PstI site found at 1121
    Illegal NotI site found at 1114
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1172
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1107
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1107
    Illegal PstI site found at 1121
    Illegal AgeI site found at 1829
    Illegal AgeI site found at 1941
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ZHANG, Fuzhong; CAROTHERS, James M.; KEASLING, Jay D. Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids. Nature biotechnology, 2012, 30.4: 354.


Source

ZHANG, Fuzhong; CAROTHERS, James M.; KEASLING, Jay D. Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids. Nature biotechnology, 2012, 30.4: 354.

References